The HPLC-Chip Cube MS InterfaceIncludes G4240A Chip cube unit, Chip Cube Interface Assembly (also referred to as Spray Chamber), and wiring harness with connectors for interface assembly. With the invention of HPLC-Chip technology, Agilent is significantly reducing the need for user interaction and making nanospray a rugged state-of-the-art technology. The cornerstone of chip technology is the HLPC-Chip, a 3-dimensional structure made of sandwiched polyimide films. Grooves of specific dimensions are laser-ablated into a layer of polyimide film. The lamination of a top and bottom layer then forms channels of trapezoidal or triangular shape inside the chip which can either be used as capillaries or, if filled with packing material, as nanocolumns. One end of the chip tapers off into a polyimide nanosprayemitter.The Chip Cube belongs to the Agilent 1260 Infinity HPLC family. It interfaces the HPLC-Chip to the pump and the μ-well-plate sampler of the nano-LC system. The Chip Cube is connected to the other Agilent 1260 Infinity modulesvia CAN bus. The main functions are:
• Connect the HPLC-Chip automatically to solvent lines and grounding contact.
• Position the sprayer tip reproducible in front of the MS inlet.
• Provide two independent, concentrically high pressure nano flow valves.
The Chip Cube Interface Assembly is effectively a spray chamber for use with the Chip Cube.